Science Advances |  October 13, 2022

1000 spider silkomes: Linking sequences to silk physical properties

Author:Kazuharu Arakawa1,2,3,4*, Nobuaki Kono1,3, Ali D. Malay5, Ayaka Tateishi5,6, Nao Ifuku5, Hiroyasu Masunaga7, Ryota Sato5,8, Kousuke Tsuchiya5,6, Rintaro Ohtoshi5,8, Daniel Pedrazzoli8, Asaka Shinohara8, Yusuke Ito8, Hiroyuki Nakamura5,8, Akio Tanikawa9, Yuya Suzuki10,11, Takeaki Ichikawa12, Shohei Fujita13, Masayuki Fujiwara1, Masaru Tomita1,2,3, Sean J. Blamires14, Jo-Ann Chuah5, Hamish Craig5,14, Choon P. Foong5,6, Gabriele Greco15, Juan Guan16, Chris Holland17, David L. Kaplan18, Kumar Sudesh19, Biman B. Mandal20,21,22, Y. Norma-Rashid23, Nur A. Oktaviani5, Rucsanda C. Preda18, Nicola M. Pugno15,24, Rangam Rajkhowa25, Xiaoqin Wang26, Kenjiro Yazawa5, Zhaozhu Zheng26, Keiji Numata5,6*


  • 1. Institute for Advanced Biosciences, Keio University
  • 2. Faculty of Environment and Information Studies
  • 3. Graduate School of Media and Governance
  • 4. Exploratory Research Center on Life and Living Systems (ExCELLS)
  • 5. Biomacromolecules Research Team
  • 6. Department of Material Chemistry
  • 7. Japan Synchrotron Radiation Research Institute
  • 8. Spiber Inc.
  • 9. Graduate School of Agricultural and Life Sciences
  • 10. Graduate School of Life and Environmental Sciences
  • 11. The United Graduate School of Agricultural Sciences
  • 12. Kokugakuin Kugayama High School
  • 13. Graduate School of Agriculture
  • 14. Evolution and Ecology Research Centre
  • 15. Department of Civil
  • 16. Beijing Advanced Innovation Center for Biomedical Engineering
  • 17. Natural Materials Group
  • 18. Department of Biomedical Engineering
  • 19. School of Biological Sciences, Universiti Sains Malaysia
  • 20. Department of Biosciences and Bioengineering
  • 21. Center for Nanotechnology
  • 22. School of Health Sciences and Technology
  • 23. Institute of Biological Sciences
  • 24. School of Engineering and Materials Science
  • 25. Institute for Frontier Materials
  • 26. College of Textile and Clothing Engineering
The balance of crystalline and amorphous regions in the fibroin structure underpins the tensile strength of bagworm silk

From “1000 spider silkomes: Linking sequences to silk physical properties” by Kazuharu Arakawa et al.. Licensed under CC BY-NC 4.0.


Spider silks are among the toughest known materials and thus provide models for renewable, biodegradable, and sustainable biopolymers. However, the entirety of their diversity still remains elusive, and silks that exceed the performance limits of industrial fibers are constantly being found. We obtained transcriptome assemblies from 1098 species of spiders to comprehensively catalog silk gene sequences and measured the mechanical, thermal, structural, and hydration properties of the dragline silks of 446 species. The combination of these silk protein genotype-phenotype data revealed essential contributions of multicomponent structures with major ampullate spidroin 1 to 3 paralogs in high-performance dragline silks and numerous amino acid motifs contributing to each of the measured properties. We hope that our global sampling, comprehensive testing, integrated analysis, and open data will provide a solid starting point for future biomaterial designs.

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本研究の遂行に際し、当社は 内閣府 革新的研究開発推進プログラム(ImPACT)「超高機能構造タンパク質による素材産業革命」 の助成を受けました。