Polymer Degradation and Stability
|
August 12, 2017

Chemiluminescence from recombinant spider silk under thermo-oxidative degradation

Authors:Koyuru Nakayama1, Anh T.N. Dao1, Kengo Takeuchi1, 2, Junichi Shimokata1,2, Toshiaki Taniike1,

DOI: 10.1016/j.polymdegradstab.2017.08.010

  • 1. Graduate School of Advanced Institute of Science and Technology, Japan Advanced Institute of Science and Technology
  • 2. Spiber Inc.
AbstractIn this work, the oxidative degradation of recombinant spider silk in a solid state was studied based on chemiluminescence analysis. It was found that the chemiluminescence behavior of the recombinant spider silk closely resembled that of aliphatic polyamide: The emission developed without any induction time, and the maximum intensity was observed in the range of 480–520 nm. In combination with infrared spectroscopy, it was concluded that the oxidative degradation of the recombinant spider silk initiates mainly through the hydroperoxide formation at the Cα position of amino acid residues, and its unimolecular decomposition produces imide or α-keto amide groups together with the CL emission.

 

 

-> Read full paper

Spiber’s research initiatives into novel protein materials have benefited from subsidies provided by the ImPACT Program on behalf of the Japanese Cabinet’s Council for Science, Technology and Innovation.

Share this paper

Latest academic papers

ACS Biomaterials Science & Engineering
|
November 19, 2024

Correlating Mechanical Properties and Sequence Motifs in Artificial Spider Silk by Targeted Motif Substitution

Author: Hiroyuki Nakamura¹ ² ³, Yusuke Ito³, Ryota Sato³, Hongfang Chi³, Chikako Sato³, Yasuha Watanabe¹, Kazuharu Arakawa¹ ² DOI: 10.1021/acsbiomaterials.4c01389 Abstract The major ampullate silk of orb-weaving spiders is renowned for its exceptional mechanical properties, including high tensile strength and extensibility. The development of artificial spider silk presents a promising alternative to traditional fibers with […]

View project →
Organic & Biomolecular Chemistry
|
May 22, 2024

Efficient synthetic methods for a,ß-dehydroamino acids as useful and environmentally benign building blocks in biological and materials science

Author:Taiki Mori1,Sao Sumida1,Kazuki Sakata2,Seiji Shirakawa1 DOI:10.1039/D4OB00507D 1. Institute of Integrated Science and Technology, Nagasaki University 2. Spiber Inc. Abstract Both natural and unnatural amino acids, peptides, and proteins are widely recognized as green and sustainable organic chemicals, not only in the field of biological sciences but also in materials science. It has been discovered that […]

View project →